Extended Data Fig. 3: ELISA screening of potential Pse-specific hybridoma supernatant.
From: Uncovering bacterial pseudaminylation with pan-specific antibody tools
(A) ELISA screen of hybridoma supernatant (1:1000) against: (i) control peptides 8, 9 and 10 conjugated to BSA (green), (ii) Pse peptides α-8, α-9 and α-10 conjugated to BSA (orange), and flagellin purified from C. jejuni 81-176, which is also pseudaminylated with Pse5Ac7Ac (blue). Data are presented as mean values ± SD for n = 2. (B) ELISA screen of hybridoma supernatants (1:2000) that are Pse-specific against: (i) α-configured Pse peptides α-8, α-9 and α-10 conjugated to BSA (orange) and (ii) β-configured Pse peptides β-8, β-9 and β-10 conjugated to BSA (blue). Data are presented as mean values ± SD for n = 2.
