Extended Data Fig. 1: C28 reversion analysis.
From: Rapid evolution of a highly efficient RNA polymerase by homologous recombination
a, Three segments of C28 harboring acquired mutations were reverted back to the parental Tgo-QGLK to create individual reversion constructs, RC1 (triangle), RC2 (square), and RC3 (diamond). RC3 contains five mutations, three of which are not observed in the crystal structure. b, Comparison of C28 activity against RC1, RC2, and RC3. Product formation was evaluated after 15, 30, or 60 s of incubation at 55 °C in an RNA primer extension assay with decreasing substrate concentrations.
