Extended Data Fig. 2: C28 activity under fidelity assay conditions.
From: Rapid evolution of a highly efficient RNA polymerase by homologous recombination
a, Schematic depicting the standard primer extension assay using a 5’ IR680-labeled primer annealed to a DNA template. b, Schematic depicting the ligation of a 5’ IR680-labeled primer to a chemically synthesized 5’ phosphorylated RNA oligonucleotide to generate an authentic standard for the product of the primer extension reaction. c, Denaturing PAGE analysis of C28 product formation after 1-h of incubation at 55 °C along with an authentic standard of primer-extended product.
