Extended Data Fig. 3: C28 extends primers despite template mismatches.
From: Rapid evolution of a highly efficient RNA polymerase by homologous recombination
A schematic depicts the 5’ IR680-labeled primer terminating in a 3’ cytosine, paired with a DNA template in which the complementary position (X) is either a matched guanine or one of the three mismatched bases (adenine, cytosine, or thymine). Comparison of C28 product formation reveals full-length product in all primer-duplex pairs following a 1-h incubation at 55 °C.
