Extended Data Fig. 4: Evaluation of the performance of soluble IDPs (sIDPs).
From: Directed evolution of functional intrinsically disordered proteins
a. The evolution landscape of evolved soluble IDPs. The panel shows fitness curves of the selected evolved soluble IDPs (sIDPs) from two rounds of evolution. selected: variants with growth curves within the blue-shaded area were chosen for further characterization. non-selected: variants with growth curves within the red-shaded area were excluded from further confocal microscopy characterizations. Selected variants were subcloned and fused with sfGFP for confocal characterization. The non-phase separable variants identified by confocal microscopy were termed sIDP1-6, respectively. n = 1. Scale bars represent 3 μm. b. Kyte-Doolittle hydropathy scores of the evolved soluble synIDPs. The hydropathy parameter is a rescaled Kyte-Doolittle hydropathy value ranging from 0 (least hydrophobic) to 9 (most hydrophobic). c. Evaluation of the expression of the TEV-sIDPs by SDS-PAGE from the soluble and insoluble fraction of the cell lysates. The arrows mark the positions of the target proteins on the bands. Prestained Kaleidoscope™ ladder was used as a molecular weight standard for all gels.
