Extended Data Fig. 1: B4 is an activity-based probe for the autopalmitoylated proteome.
From: Autopalmitoylation of IDH1-R132H regulates its neomorphic activity in cancer cells
a-b, Comparison of labeling efficiency of different probes in HEK293A lysate (a) or live cells (b). A21, A63, B1, B4: 10 µM; alkyne 2-BP: 50 µM. c, Competition of B4 (0.1 µM) labeling of His-TEAD2 by palmitoyl-CoA. d, Inhibition of His-TEAD2 autopalmitoylation by non-tag-B4. Alkyne palmitoyl-CoA: 1 µM. e, Competition of B4 (0.1 µM) labeling of His-Bet3 by palmitoyl-CoA. f, Inhibition of His-Bet3 autopalmitoylation by non-tag-B4. Alkyne palmitoyl-CoA: 1 µM. g, Mutation of C359 blocked the labeling of Myc-TEAD1 by B4 (10 µM) in HEK293A cells. h, Labeling of endogenous ZDHHC7 by B4 (10 µM) in HEK293A cells. i, Mutation of C160 inhibited the labeling of HA-ZDHHC7 by B4 (10 µM). j-k, Gel-based profiling of autopalmitoylated proteome with B4 (10 µM) in HEK293A cells (j) or lysate (k) at different time points. The experiments in a-k were repeated independently three times with similar results and representative results are presented.
