Extended Data Fig. 2: LC-MS analysis of IVT products with template TC.
From: Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase
Extracted ion chromatograms of IVT products with and without addition of NpnNs. IVT products encoded from the +1 TSS are marked blue, IVT products encoded from the +1 TSS with an extra C added at the 3′ end are marked dashed blue. IVT products encoded from the −1 TSS are marked red, IVT products encoded from the −1 TSS with an extra C added at the 3′ end are marked dashed red. The sequences of the formed products with exact masses are shown in Supplementary Table 3. The PAGE gel from Fig. 1d is reproduced here for clarity (see Fig. 1c-e legend). Created in BioRender. Serianni, V. (2025) https://BioRender.com/55wowug.
