Extended Data Table 1 Sequences of template and non-template DNA strands used to construct artificial DNA promoters with pre-formed transcription bubbles

From: Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase

  1. The sequences have various combinations of nucleotides at the −1 and +1 positions (indicated by capital letters in the template names while the −2 position is shown in lower-case). The aTT template is shortened by one nucleotide. NT strand = non-template strand 5′ to 3′; T = template strand 3′ to 5′. The +1 consensus transcription start sites are bold. DNA templates used for transcription and cryo-EM analyses were prepared by annealing NT and T strands with the same name, except for tTC (T strand, Supplementary Table 1), which was annealed with the TC (NT strand). Pre-formed transcription bubbles are underlined.
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