Extended Data Fig. 10: α-sintide improves cytotoxic cytokine production by chronically activated CD8+ T cells.
From: Inhibitory probes for spatiotemporal analysis of Gαs protein signaling
Diagram on the left represent the experimental design to assess the effect of α-sintide on T cells. Chronically activated human CD8+ T cells were treated with 30 µM of α-sintide WT (orange) or α-sintide W11 A (blue) for 1 day before analysis. Graphs in the middle correspond to the percentage of CD8+ T cells positive for IL-2 and IFNγ (IL-2+/ IFNγ+, top graph), or for IL-2 and TNFα (IL-2+/ TNFα+, bottom graph) quantified by flow cytometry. Bar graphs are mean ± S.E.M. (n = 3 independent experiments), ns, p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA corrected for multiple comparisons (Dunnett). Pseudocolor plots on the right display the average distribution of CD8+ T cells across all replicates. The flow cytometry plots corresponding to the DMSO conditions are reproduced from Fig. 6d because these were the same controls for experiments presented in the two figures.
