Extended Data Fig. 7: CIRCLE-seq analyses for T3 and EMX1 gRNAs after ØXØ modification.
From: Abasic CRISPR RNAs inherently harness fidelity of SpCas9 for genome editing
a, b, Comparative analyses of CIRCLE-seq off-target sites between unmodified and ØXØ gRNAs as in Extended Data Fig. 6g, h except for T3 (50 nM); Venn diagrams (a) and bar plots (b). c, CIRCLE-seq results from ØXØ and unmodified gRNA (T3 at 50 nM) for validated off-targets in targeted sequencing (Fig. 4g, i) as analyzed in Extended Data Fig. 6i. Results from CIRCLE-seq experiments with unmodified or ØXØ gRNA targeting VEGFA (T3 at 50 nM). Confident CIRCLE-seq sites (n = 140 sites; P < 0.01 relative to mismatch score = 6) are shown. Sites are displayed as absent (grey) or with reduced normalized cleavage rates (“norm”, %), based on normalized read counts (“reads”; reads per million mapped reads, RPM) relative to the on-target site, and scaled to match indel ratios (%) from targeted sequencing (Fig. 4f); the T3 on-target site is shown in red.
