Extended Data Fig. 1: Recruitment of an MCAK catalytic mutant to kinetochores modestly shortens the metaphase spindle. | Nature Chemical Biology

Extended Data Fig. 1: Recruitment of an MCAK catalytic mutant to kinetochores modestly shortens the metaphase spindle.

From: Microtubule depolymerization at kinetochores restricts anaphase spindle elongation

Extended Data Fig. 1: Recruitment of an MCAK catalytic mutant to kinetochores modestly shortens the metaphase spindle.The alternative text for this image may have been generated using AI.

MCAK depolymerase activity was suppressed by generating a mutant (MCAKMUT) with combined mutations at S192E and the hypir triple substitution (H536A/R540A/K543A). Pole-to-pole distances were measured for each cell and averaged at every time point (n = 29 MCAK cells pooled from four independent experiments; n = 8 MCAKMUT cells pooled from two independent experiments). For MCAK cells, data are replotted from Fig. 1f. Error bars: mean ± SEM.

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