Extended Data Fig. 8: Vesicular transported GPR56 activate multiple G protein signaling in the recipient cells.
From: Adhesion GPCR-induced ectocytosis mediates intercellular GPCR signal propagation
a, Representative confocal images of HEK293T cells expressing Gαq-GFP (green) alone, or expressing Gαq-GFP (green) with Halo-GPR56 (magenta), or expressing Gαq-GFP (green) treated with purified migrasomes (magenta) derived from HEK293GPR56-mCherry cells. Scale bar, 10 μm. Fluorescence intensity profiles along the indicated lines are shown on the right. Images are representative of >30 cells from three biologically independent experiments. b, BRET-based assessment of Gq activation in response to migrasome treatment, measured using the Gq-CASE biosensor. Data are presented as mean ± SEM from three biologically independent experiments, each performed in triplicate. Statistical significance was determined by ordinary one-way ANOVA with Tukey’s test. PBS versus Migs, **P = 0.0016; Migs versus (Migs + DHM), **P = 0.0030. c, HEK293T cells co-expressing SRF-RE with PM-p115 or Endo-p115, were co-transfected with GPR56 for 24 hours, followed by luciferase assay. Data are presented as mean ± SEM from three biologically independent experiments, each performed in triplicate. Statistical analysis was performed by ordinary one-way ANOVA with Tukey’s multiple comparisons test. ****P < 0.0001.
