Extended Data Fig. 1: GPR56 induce the biogenesis of matrix-bound migrasomes and retractosomes.
From: Adhesion GPCR-induced ectocytosis mediates intercellular GPCR signal propagation
a, Long-term live-cell imaging of HEK293 cells expressing GPR56-Venus, cultured on collagen-coated glass-bottom dishes, related to Supplementary Video 1. Scale bar, 10 μm. b, HEK293GPR56-mCherry cells were cultured on collagen-coated glass-bottom dishes and imaged by structured illumination microscopy (SIM). Scale bar, 10 μm. Insets show enlarged regions of interest (ROIs). White arrows in the top panels indicate the migrasomes, white arrows in the bottom panels indicate the retractosomes. c, Representative confocal images of HEK293GPR56-mCherry cells cultured on collagen-coated, Fibronectin-coated or non-coated glass-bottom dishes. Scale bar, 10 μm. Insets show enlarged ROIs. Scale bar, 10 μm.
