Extended Data Fig. 2: Spatial organization of GPR56 in retraction fibers and migrasomes.
From: Adhesion GPCR-induced ectocytosis mediates intercellular GPCR signal propagation
a-b, Representative confocal images of HEK293 cells co-expressing GPR56-Venus with ITGA5 (integrin α5)-mCherry (a) or TSPAN4-mCherry (b). Insets show enlarged regions. Scale bars, 10 μm. Images are representative of > 20 cells from three biological independent experiments. c, Representative confocal images of HEK293 cells co-expressing GPR56-Venus and TSPAN4-mCherry. Insets show enlarged regions of interest (ROIs). Scale bar, 10 μm. Images are representative of > 30 cells from three biologically independent experiments per condition. The white arrows indicate the migrasomes labeled by GPR56 but not TSPAN4. d, Representative TIRF-SIM images of HEK293 cells co-expressing GPR56-Venus and TSPAN4-mCherry. Scale bar, 10 μm. per condition. Insets show enlarged regions of interest (ROIs). Scale bar, 10 μm. Images are representative of > 30 cells from three biologically independent experiments per condition. The white arrows indicate the migrasomes labeled by GPR56 but not TSPAN4.
