Fig. 3: Hetero-oligomerization is inducible in yeast and promotes decamer stabilization.
From: Hetero-oligomerization drives structural plasticity of eukaryotic peroxiredoxins
a, Graph showing the GFP fluorescence intensity of roGFP2-Tsa1 and roGFP2-Tsa2 constructs in yeast cells at the indicated time points after the treatment with 1 mM exogenous H2O2. The constructs were expressed from genes integrated into the TSA1 and TSA2 genomic loci, respectively, remaining under the control of the native promoters and 3′ untranslated regions. Experiments were repeated thrice with independent yeast cultures. Data represent mean ± s.d. b, Clear native PAGE gels, monitored for GFP fluorescence, of lysates taken from the cultures of the indicated yeast strains at the indicated time points following the addition of 1 mM exogenous H2O2. Wedges indicate hetero-oligomers containing an increasing number of Tsa2 subunits (first panel from the left) and an increasing number of roGFP2-Tsa2 subunits (third panel from the left). Two further experimental repeats are shown in Supplementary Fig. 10.
