Extended Data Fig. 3: Single particle cryo-EM data collection and processing for ScALG3, HsALG9 and GgALG12 structures. | Nature Chemical Biology

Extended Data Fig. 3: Single particle cryo-EM data collection and processing for ScALG3, HsALG9 and GgALG12 structures.

From: Structures of ALG3/9/12 reveal the assembly logic of the N-glycan oligomannose core

Extended Data Fig. 3: Single particle cryo-EM data collection and processing for ScALG3, HsALG9 and GgALG12 structures.The alternative text for this image may have been generated using AI.

Panels a-f pertain to the ALG3-ternary complex. a, Representative micrograph. b, Reference-free 2D class averages. c, Processing strategy in cryoSPARC82. d, Gold standard half-map and model-map FSC curves shown in black and blue lines, respectively. The dashed lines are shown at 0.143 and 0.5 FSC. e, Final map coloured by local resolution. f, Particle orientation distribution plot. Panels g-l pertain to the ALG9-first-ternary complex and are listed in order as panels a-f. Panels m-r pertain to the ALG12-ternary complex and are listed in order as panels a-f. The scale bar in panels a, g and m represents 100 nm.

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