Extended Data Fig. 5: Tracking individual heavy-chain BCR lineages before and after LAIV stimulation in tonsil organoids show evidence of isotype switching.
From: Modeling human adaptive immune responses with tonsil organoids
Immunoglobulin heavy-chain gene rearrangements for each isotype were sequenced from total memory B cells, germinal center B cells, and plasmablasts sorted from cultures of four donors on days 0 and 7. Heavy-chain BCR sequences from lineages that contained members as only IgM on day 0 and as only isotype-switched on day 7 were compared for their somatic hypermutation levels. For each lineage, the mean SHM was calculated for day 0 IgM members and for day 7 switched members, and the difference between these means was plotted. Lineages with increased mutation are shown in blue and those with decreased mutation in red.
