Extended Data Fig. 4: Mapping of human-AAV chimeric reads against the vector plasmid. | Nature Medicine

Extended Data Fig. 4: Mapping of human-AAV chimeric reads against the vector plasmid.

From: Contaminating plasmid sequences and disrupted vector genomes in the liver following adeno-associated virus gene therapy

Extended Data Fig. 4: Mapping of human-AAV chimeric reads against the vector plasmid.

A) DNA and B) RNA chimeric read alignment showing sequence coverage plotted as the frequency each nucleotide position appears in chimeric reads along the vector reference plasmid (x-axis). The plasmid map above each graph illustrates the corresponding functional elements. Key components include: M13 ori (bacteriophage origin of replication), ITR (inverted terminal repeat), CMV (cytomegalovirus enhancer), CBA (chicken beta-actin core promoter), SV40 intron (simian virus 40 small intron), SMN (spinal motor neuron coding sequence), SMN 3’UTR (noncoding region from endogenous SMN locus), bGH polyA (bovine growth hormone polyA signal), AmpR promoter (bacterial promoter for ampicillin resistance), KanR (kanamycin resistance marker), and ori (bacterial origin of replication).

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