Extended Data Fig. 7: Single-cell analysis of matched peripheral blood and CSF samples in patients with neuro-CirAE (n = 4).

(a) Uniform Manifold Approximation and Projection (UMAP) visualization of CART cells in peripheral blood (PB) and cerebrospinal fluid (CSF), colored by T-cell clusters in each of the samples. (b) AUCell enrichment score for Gene Ontology Biological process (GOBP): leukocyte-mediated cytotoxicity score in each of the clusters. Box plots show the median (center line), interquartile range (bounds of box; 25th–75th percentiles), and minimum and maximum values (whiskers). Statistical comparisons between groups were performed using two-tailed Wilcoxin test. (c) Density plot of representative genes involved in T-cell cytotoxic function (Granzyme K,B,A: GZMK,GZMB,GZMA; Granulysin: GNLY; interferon gamma: IFNG; perforin 1: PRF1; CD107a) in PBMC and CSF samples.