Fig. 6: Descartes-08 remodels the TCR repertoire without inducing major changes to the T cell compartment.
a,b, TCR repertoire profiling of participants assigned to placebo (a) and DC-08 (b) cohorts comparing day 1 baseline and month 2. Scatter plots showing frequencies of individual TCR-β sequences in peripheral blood. Dotted and dashed diagonal lines show parity and two-fold changes. Significantly expanded or contracted TCR-β clonotypes are indicated with blue and yellow color, respectively. Significance is determined by a fold change greater than 2 and FDR-adjusted P < 0.01. For DC-08-treated participants, clonotypes found in DC-08 product are indicated with a thick outline. c,d, Frequencies of expanded (c) and contracted (d) clonotypes in placebo (n = 5) and DC-08 (n = 10) participants. Dataset was computationally downscaled to 100,000 templates to control for variation in sequencing depth between samples. e–m, Comparative frequencies of indicated T cell subsets (including Th1, Th2 and Th17 (e–g), along with CD4+ and CD8+ TSCM (h,k), TCM (i,l) and TEM (j,m)) in PBMCs at month 1 and month 3 expressed as fold change from SCR. PBMCs were analyzed from anti-AChR antibody seropositive participants for placebo (n = 10) and DC-08 (n = 11). In c and d, P values were generated by square root transformation of the data and a two-sided unpaired t-test with Welch’s correction. In e–m, y axes are log2 scale, and P values were generated by log transformation of data and two-sided multiple t-tests with correction for multiple comparisons (Benjamini, Krieger, Yekutieli with a 5% FDR). Box-and-whisker plots show the median, interquartile range and full range of data, along with individual data points. Source data are provided for this figure.
