Extended Data Fig. 4: In vitro mechanism of resistance against phage øSimón. | Nature Medicine

Extended Data Fig. 4: In vitro mechanism of resistance against phage øSimón.

From: Cross-reactive anti-prophage antibodies and bacterial heteroresistance implicated in phage therapeutic failure

Extended Data Fig. 4: In vitro mechanism of resistance against phage øSimón.The alternative text for this image may have been generated using AI.

a: Representation of the btuB gene sequence in wt B. bronchialis (top) and a mutant resistant to øSimón (bottom). The red arrow and box indicate the insertion of an alanine at position 599, leading to a premature stop codon in the phage-resistant bacterium. b: Bacterial growth curves of wt B. bronchialis and the BtuB-deficient variant presented in panel (a), with and without phage (mean ± SD; n = 3 biological replicates). cand d: Bacterial growth curves of wt and BtuB-deficient B. bronchialis, respectively, in serial 1:2 dilutions of heart infusion (HI) broth in PBS (mean ± SD; n = 3 biological replicates). e: For the dilutions from panels (c) and (d) that supported B. bronchialis growth, comparison of the area under the curve between wt (grey) and BtuB-deficient (pink) isolates (bars are medians; n = 3 biological replicates; 2-way ANOVA; two-tailed). ns: not significant. f: Adsorption assay of øSimón to wt or BtuB-deficient B. bronchialis over 90 minutes (median ± 95% CI; n = 3 biological replicates; Kruskal-Wallis test).

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