Extended Data Fig. 3: øSimón neutralisation is mediated by anti-øSimón antibodies.
a: Correlation between the number of successive passages of serum on øSimón-coated wells, and the ELISA signal for anti-øSimón antibodies (n = 2 biological replicates). Simple linear regression with 95% CI shaded zones depicted in magenta. Using the equation from the regression (top right), it was predicted that the signal for anti-øSimón antibodies would reach the level of the vehicle control after 10 passages. b: Neutralisation assay of a 106 pfu/ml dose of øSimón after 60 min of incubation in day 0 patient serum 1:100 in PBS (black circles), or the same serum sample after 12 passages for anti-øSimón antibody depletion (magenta squares). The values for the postdepletion column were corrected by subtracting the amount of residual øSimón from the passages used for antibody depletion. Bars are medians ± 95% CI; n = 4 biological replicates; Mann-Whitney test; two-tailed. c: Bactericidal effect of patient serum, control serum or a PBS control over a B. bronchialis population. Bacteria were counted before (black circles) and after (magenta triangles) 30 minutes of treatment (bars are medians; n = 3 biological replicates; two-tailed two-way ANOVA: ns: not significant). d: The bacterial populations from panel (c) were used in an adsorption assay with øSimón. There were no differences in øSimón’s ability to adsorb to any of these bacterial cells after 80 minutes of coincubation (median ± 95% CI; n = 3 biological replicates).
