Extended Data Fig. 3: List of human organs and biological samples compatible with this method and the preparation time for each step. | Nature Protocols

Extended Data Fig. 3: List of human organs and biological samples compatible with this method and the preparation time for each step.

From: Preparation of large biological samples for high-resolution, hierarchical, synchrotron phase-contrast tomography with multimodal imaging compatibility

Extended Data Fig. 3

The process described in this protocol paper should work for all major organs, but only the organs listed here have been tested. The maximum degassing times listed here are specific to our vacuum degassing setup and are subject to change depending on the pump, the volume to be pumped, the pumping section, the quantity of gas to be evacuated, and the paths that the gas can take to leave the sample. As such, they should be adapted to each user vacuum setup by looking at the bubbling intensity as explained in Step 4A(ii) of the protocol.

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