Fig. 3: Step-by-step description of a stress test.
From: OBSERVE: guidelines for the refinement of rodent cancer models
a,b, A stress-test should be performed by restraining the mouse as demonstrated in a and pressing the xyphoid with the finger of the dominant hand as demonstrated in b. Gentle-to-moderate pressure should be applied for 3 s. The red circle in a indicates the xyphoid where pressure should be applied. The arrows in b and c indicate the important features when holding the mice.
