Correction to: Scientific Reports https://doi.org/10.1038/s41598-023-29157-z, published online 01 March 2023
Figure 1 in the original version of this Article displayed a yellow border which was redundant and consequently removed.
The original Figure 1 and accompanying legend appear below.
Effect of sortilin depletion on the distribution and processing of Cathepsin D in RCE-1 cells. (A) Immunofluorescence staining of sortilin and CatD in the RCE1 cells. Confocal representative images and quantification of co-localization of sortilin and CatD (MCC-M1); CatD and sortilin (MCC-M2). The shown cells were taken from the framed area of the lower magnification image (right merge). (B) Representative immunofluorescence staining of CatD and LAMP-1 in RCE1 and RCE-1 KD cells. Quantification of co-localization of LAMP-1 and CatD (MCC-M1); CatD and LAMP-1 (MCC-M2). Values are expressed as the means of Manders colocalization coefficients 1 and 2 (MCC-M1 and MCC-M2, respectively) ± SEM. (*) significant difference from RCE-1 (p < 0.01). Scale bars = 10 μm. (C) Representative immunoblot of CatD in RCE-1 M (mock-depleted), RCE-1 and RCE-1 KD cells. Bands intensities of pro-CatD (52 kDa) and CatD (48 kDa) were quantified separately. Bars represent the means of the total CatD (pro-CatD + CatD), as relative optical densities (R.O.D.) ± SEM (upper histogram) and the means of pro-CatD percentage in each sample ± SEM (lower histogram) from four independent experiments. (*) and (**) significant differences (p < 0.01 and p < 0.05, respectively). Ponceau S staining were used as loading control. The full-lenght immunoblot is presented in Supplementary Fig. 2.
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Aguilera, A.C., Leiva, N., Alvarez, P.A. et al. Publisher Correction: Sortilin knock-down alters the expression and distribution of cathepsin D and prosaposin and up-regulates the cation-dependent mannose-6-phosphate receptor in rat epididymal cells. Sci Rep 13, 4152 (2023). https://doi.org/10.1038/s41598-023-31255-x
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DOI: https://doi.org/10.1038/s41598-023-31255-x
