Fig. 3: Muscleblind family gene expression analysis.
From: Use of HSALR female mice as a model for the study of myotonic dystrophy type I
a, Mbnl1 gene expression, measured by qRT–PCR, is significantly increased in HSALR muscles compared with WT. b, By contrast, Mbnl2 expression is reduced in HSALR muscles. c, MBNL1 protein levels, as measured by quantitative dot blot, show a significant decrease in HSALR muscles compared with WT. d, miR-218 levels are increased in the muscles of HSALR animals compared with WT. The boxes indicate the two central quartiles, the midline represents median values and the whiskers indicate the minimum and maximum data value, excluding outliers. e,f, Representative western blot membranes depicting MBNL1 protein levels in quadriceps (e) and gastrocnemius (f). Statistical analysis was performed using two-way ANOVA (black asterisks) ***P < 0.001; *P < 0.05. Sample sizes for the whole figure: n = 10, n = 15, n = 17 and n = 24 for WT males and females and HSALR males and females, respectively. Quadriceps and gastrocnemius were analyzed for each individual.
