Fig. 2: Generation of the first transgenic G. mellonella reporter line expressing both GFP and DsRed.
From: PiggyBac-mediated transgenesis and CRISPR–Cas9 knockout in the greater wax moth, Galleria mellonella
a, The helper and donor constructs used to develop the Bmhsp90:GFP/3xP3:DsRed transgenic strain are represented. b, Both eGFP and DsRed expression can be observed in the late embryo (top row; G4 animals), first instar larvae (middle row; G2 animals) and fourth instar larvae (bottom row; G4 animals). eGFP expression is very bright within the unmelanized tissues, while DsRed expression is limited to neural tissue including the brain, optic nerves and stemmata. c, The transgenic cassette appears to be inserted within an inorganic phosphate cotransporter gene cluster on chromosome 12 in the intergenic space between LOC113515287 and LOC113512789, adjacent to a small cluster of putative (put.) odorant-like receptors.
