Fig. 3: Generation of GFP-tubulin and histone-mCherry reporter lines.
From: PiggyBac-mediated transgenesis and CRISPR–Cas9 knockout in the greater wax moth, Galleria mellonella
a, Constructs used to generate transgenic lines Gmhsp90:GFP-αtub1b and Bmhsp90:his2av-mCh. b, Gmhsp90:GFP-αtub1b larvae (top left) with fat body tissue fixed and stained with an anti-GFP antibody (top right); and Bmhsp90:his2av-mCh (bottom left) larvae with fat body and dorsal neural ganglion imaged live (bottom right). Zoomed panels are not images from the same larvae. Expected cytoskeletal distribution of eGFP was observed, corresponding with expected localization of tubulin, while a nuclear localization was observed for mCherry. c, Brightfield (BF), mCherry and eGFP tissue expression patterns in a strain with both Bmhsp90:his2av-mCh/Gmhsp90:GFP-αtub1b expression cassettes. Strong fat body expression was observed for both fluorophores, with the G. mellonella hsp90 promoter that seemed to drive strongest expression in gut and silk gland, while the B. mori hsp90 promoter was stronger in epidermal and muscle tissue (not shown), but very weak in silk glands and Malpighian tubules.
