Fig. 5: Activity analysis of group-housed male and female AFO mice using the IntelliProfiler workflow.
a–l, Cumulative travel distances measured in 2-h intervals for 2 days (a–c) and for 3 days (d–f) and over 12-h intervals for 2 days (g–i) and for 3 days (j–l). Analysis of parental aging effects in locomotor activity, comparing groups of 8 and 15/16 male AFO and control mice (a,d,g,j), groups of 8 and 16 female AFO and control mice (b,e,h,k), groups of 8 and 16 male and female AFO mice (c,f,i,l). m–t, Heatmaps showing relative locomotor activity. For 2-h bins, control groups included 8 mice (m) and 15/16 mice (q), and AFO groups included 8 mice (o) and 16 mice (s). For 12-h bins, control groups included 8 mice (n) and 15/16 mice (r), and AFO groups included 8 mice (p) and 16 mice (t). Values in the bar or line graphs are presented as mean ± s.d., with box plots displaying minimum and maximum whiskers. Statistical analysis was conducted using Holm–Šidák method for multiple comparisons. Significance levels are indicated as *P < 0.05, **P < 0.01 and ***P < 0.001.
