Fig. 6: The spatial relationships among group-housed male and female AFO mice using the IntelliProfiler workflow.
a, Definition of the spatial relationships between two individual mice. When another mouse is in the same grid as the focal mouse (both depicted in yellow), the relationship is defined as ‘Same’. If the other mouse is in the surrounding grids (depicted in olive), it is defined as ‘Close’. If the other mouse is outside the surrounding grids (depicted in indigo), it is defined as ‘Intermediate’. If the other mouse is beyond the ‘Intermediate’ zone (depicted in blue), the relationship is categorized as ‘Away’. b–e, The 2-h bin transition of proportions based on ‘Same’, ‘Close’, ‘Intermediate’ and ‘Away’ for groups of 8 male control mice (b), 8 female control mice (c), 8 male AFO mice (d), 8 female AFO mice (e), 15 male control mice (f), 16 female control mice (g), 16 male AFO mice (h) and 16 female AFO mice (i). b–i, The average proportion of distance categories during the light period and the dark period is shown for each group. Chi-square P values comparing the proportions between light and dark periods, P = 0.359 (b, 8 male control mice), P = 0.215 (c, 8 female control mice), P < 0.001 (d, 8 male AFO), P < 0.001 (e, 8 female AFO), P = 0.114 (f, 15 male control mice), P = 0.007 (g, 16 female control mice), P = 0.050 (h, 16 male AFO), P < 0.001 (i, 16 female AFO).
