Fig. 7: Close contact between two individuals among group-housed male and female AFO mice analyzed using the IntelliProfiler workflow.
a, Definition of close contact: two mice either in either ‘Same’ (depicted in yellow) or in adjacent grids, classified as ‘Close’ (depicted in olive). b–m, Average CCR measured in 2-h intervals (b–g) and 12-h intervals (h–m). In b, e, h and k, analysis of CCR based on group size, comparing groups of 8 and 15/16 male AFO or control mice. In c, f, i and l, analysis of CCR based on group size, comparing groups of 8 or 16 female AFO and control mice. In d, g, j and m, analysis of CCR based on group size, comparing groups of 8 or 16 male AFO and female AFO. n–u, Heatmaps showing the time course of CCR. For 2-h bins: control groups included 8 mice (n) and 15/16 mice (r) and AFO groups included 8 mice (p) and 16 mice (t). For 12-h bins: control groups included 8 mice (o) and 15/16 mice (s), and AFO groups included 8 mice (q) and 16 mice (u). Values in the bar or line graphs are presented as mean ± s.d., with box plot showing minimum and maximum whiskers. The same statistical tests (two-way ANOVA followed by Holm–Šidák’s multiple comparison test) were applied to all bar and line graphs and box plots in b–m. Statistical analysis was conducted using the Holm–Šidák method for multiple comparisons. Significance levels are indicated as *P < 0.05, **P < 0.01 and ***P < 0.001. P < 0.001 (c), P = 0.338 (d), P = 0.072 (e), P < 0.001 (f), P = 0.007 (g), P = 0.109 (h).
