Scheme 7
Conjugation of oxazolinosenes to cysteine in cells and proteins. A H1299 cells were treated with different concentrations of compounds for 72 h, cell viability was measured using a CCK-8 kit, and the IC50 value of each compound was calculated by GraphPrism software. B H1299 cells were treated with DMSO, 3A, 3A3, 6A, or cinnamaldehyde (Cin) for 3 h at a final concentration of 100 μM. The relative levels of GSH in each sample were determined with a GSH and GSSG Assay Kit, and the data are presented as the mean ± S.D. (n = 3, *p < 0.05, **p < 0.01 compared with the control; #p < 0.05, ##p < 0.01, compared with the 3A group, t-test). C The workflow for the identification of glutathione-compound conjugates in vitro and in vivo (Supplementary Fig. 12). D Modification of the free cysteine residue on BSA (Supplementary Fig. 11).
