Fig. 1: The p53 family phase behavior at the microscale resolution as a function of protein concentration and temperature.
From: Oncogenic p53 triggers amyloid aggregation of p63 and p73 liquid droplets
Bright-field differential interference contrast (DIC) microscopy images were collected, revealing (a) the concentration-dependent demixing of p63C and p73C, and (b) the demixing of p53C from the PEG solution. c The temperature dependence of p53C, M237I, p63C, and p73C demixing and aggregation (arrows). d The time dependence of p53C aggregation from droplets at 25 °C (see methods); Schematic representations of (e) p63C and p73C demixing concentrations, and (f) the temperature dependence of the studied proteins. Colored circles indicate the phase separation of p53C (green), M237I (gray), p63C (red), and p73C (blue). Circles filled with an asterisk depict aggregation conditions.
