Fig. 6: Enzymatic control over temporal chemoselective modulation. | Communications Chemistry

Fig. 6: Enzymatic control over temporal chemoselective modulation.

From: Nucleotide-mediated modulation of chemoselective protein functionalization in a liquid-like condensed phase

Fig. 6

a Schematic diagram describing the temporal regulation of NBD-functionalization of BSA in a CTAB-mediated condensate by ATP-hydrolyzing enzyme alkaline phosphatase (ALP). b Representative confocal images of the pyranine-containing BSA droplet in the presence of fixed concentration of ATP and two different concentrations of ALP (50 and 600 nM), taken after 5, 15 and 60 min. c The pH of pyranine-containing BSA droplet with ATP and different concentrations of ALP, as determined from the fluorescence intensity of confocal images. Data are presented as mean ± standard deviation (SD) (n = 10). d Change in λ[K(NBD)] as a function of time for BSA droplets containing ATP in the presence of different concentrations of ALP. Data are presented as mean ± standard deviation (SD) (n = 3). Experimental condition: [CTAB] = 1 mM, [BSA] = 50 µM, [ATP] = 200 µM, [Pyranine] = 2 µM, [NBD-Cl] = 100 µM, [HEPES] = 1 mM; pH 7.0; T = 25 °C.

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