Fig. 2: In vivo analysis of lactacystin biosynthetic gene cluster. | Communications Chemistry

Fig. 2: In vivo analysis of lactacystin biosynthetic gene cluster.

From: Biosynthesis of lactacystin as a proteasome inhibitor

Fig. 2

a Biosynthetic gene cluster of lactacystin. The cluster contains lctA, PKS-NRPS (ACP, acyl carrier protein; KS, ketosynthase; AT, acyltransferase; C, condensation domains); lctB, NRPS (A, adenylation; PCP, peptidyl carrier protein domains); lctC, cyclase; lctD, cytochrome P450; lctE, MbtH-like protein (MLP); and lctF, formyltransferase. b LC-ESI-MS analysis (positive ion mode, monitored at m/z 377 (black) and 361 (blue)) of (i) lactacystin standard and broths of (ii) the wild-type strain and (iii) the ∆lctD strain. Traces monitored at m/z 377 were enlarged 25-fold vertically. A peak (*) with mass of 361.1439 corresponding to 7 (calculated mass, 361.1428) was detected (Fig. S5). c LC-ESI-MS analysis (positive ion mode, monitored at m/z 377) of (i) lactacystin standard and broths of transformants harboring (ii) lctT to lctF, (iii) lctA to lctF, and (iv) the empty vector. d LC-ESI-MS analysis (positive ion mode, monitored at m/z 377) of (i) lactacystin standard and broths of transformants harboring (ii) lctT, B, C, D, E, F, and ACP1-inactivated lctA (S45A) and (iii) lctB, C, D, E, F, and AT1-inactivated lctA (S791A).

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