Fig. 1: Time-dependent SEC results of SOD1 aggregation.

a Size exclusion chromatogram of EDTA and DTT-treated SOD1(30 μM) at different times (t = 10, 15, and 30 min), b fitting of decrease in dimer area with for first order (Eq. 6), c the time evolution of soluble species observed between 6 and 18 ml, d at lower elution volume (6–13 ml).