Fig. 4: Different concentrations of aggregation SOD1 for the kinetics analysis. | Communications Chemistry

Fig. 4: Different concentrations of aggregation SOD1 for the kinetics analysis.

From: Biophysical and spectroscopical insights into structural modulation of species in the aggregation pathway of superoxide dismutase 1

Fig. 4

a Aggregation of different concentrations of SOD1 in 2 mM EDTA and 80 mM DTT at 37 °C, 25 mM phosphate buffer pH 7.4, b EDTA and DTT induced fibrillation of SOD1 alone and in the presence of seeds. c log-log plot of aggregation rate vs. SOD1 concentration. d–h Different concentrations of SOD1 aggregation early time rise of the ThT fluorescence intensity fits well with the relation Δ = A(cosh(bt)-1) referring to the presence of secondary pathways. i Calculation of the secondary nucleus size from the parameter κ.

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