Table 1 Melting temperatures of DNA duplexes with terminal capping

From: An epimer of threose nucleic acid enhances oligonucleotide exonuclease resistance through end capping

5’-d(ACGGCTATTGAGT)-3’

3’-d(Z TGCCGATAACTCA Z)-5’

Tma/ΔTmb (°C)

Z

DNA-T

TNA-T

INV-T

eTNA-T

5’ dangling Z

60.7/+0.2

61.5/+1.0

61.4/+0.9

61.1/+0.6

3’ dangling Z

61.2/+0.7

60.8/+0.3

62.1/+1.6

61.3/+0.8

5’ and 3’ dangling Z

61.2/+0.7

62.4/+1.9

61.5/+1.0

62.5/+2.0

None dangling

60.5

  1. aMeasured at 260 nm with 2.5 µM + 2.5 µM single-strand concentration at a heating rate of 0.6 °C/min in 1 M NaCl, 10 mM MgCl2, pH 7.0. Tm values were calculated from the heating curves.
  2. b∆Tm as calculated as Tmmodified duplex - Tmunmodified duplex.
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