Fig. 6: Quantification of analyte paracetamol with the dilution method at low magnetic field.
From: Photo-CIDNP for quantification of micromolar analytes in urine
a Calibration curves for paracetamol in range 60–200 μM (before dilution, so 3–10 μM after dilution), in three different urines. The indicated LOQs correspond to SN = 3 in native urine samples. RSDs are calculated for the points with the lowest analyte concentration (60 μM before dilution). All points were measured in triplicates using W5 water suppression, 1 s of laser irradiation, and 32 scans at 1.9 T. b 1D 1H NMR spectra at five different paracetamol concentrations for samples diluted to 5% urine V with the signal used for analysis indicated by the dashed line. c Correlation of the experimentally determined with the true concentration in three different urines I–III. The difference between the true and the determined concentration is indicated next to each point with the percentage error in the brackets.
