Fig. 2: Photostability of dyes attached in different protein secondary structures.
From: Photostability of organic fluorophore influenced by adjacent amino acid residues
A Three labeling sites of EF-G. E205, G239, and E308 were mutated to Cys, respectively, for dye labeling. Total photon count, bleaching time, and intensity of Cy3 (red) and Cy5 (blue) attached at EF-G-E205C (B), EF-G-G239C (C), and EF-G-E308 (D). Abbreviations of EF-G variants in the figures are named after the residues adjacent to the fluorophore. The standard deviation of three repeats is shown as the error. Each experimental condition comprises analysis of no fewer than 800 single-molecule traces.
