Fig. 2: H93 inhibits the proliferation of prostate cancer (PCa) cells in vitro. | Communications Chemistry

Fig. 2: H93 inhibits the proliferation of prostate cancer (PCa) cells in vitro.

From: A Hybrid compound H93 treats prostate cancer by directly binding UHRF1 and promoting protein dimerization

Fig. 2

a Colony formation assays were used to test the inhibitory activity of H93 in human umbilical vein endothelial cells (HUVEC) or PCa cells. Experiments were performed in triplicate. (Upper) Representative images of colony formation assays are presented. (Lower) The half-maximal inhibitory concentration (IC50) values of each cell line are shown as mean ± SD. b UHRF1 protein expression in HUVEC and PCa cells was assessed by immunoblotting. Experiments were performed in triplicate. c The correlation between H93 inhibitory activity and UHRF1 expression levels in PCa cells was analyzed. A scatter plot illustrates the relationship between relative UHRF1 protein levels and IC50 values. IC50 values were determined using a dose-response nonlinear regression model, and Pearson’s correlation coefficient (r) was calculated via linear regression.

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