Fig. 6: Chemical synthesis of ubiquitin (1-76) based on VTANCL. | Communications Chemistry

Fig. 6: Chemical synthesis of ubiquitin (1-76) based on VTANCL.

From: Rapid vinyl thianthrenium tetrafluoroborate-promoted thioacid-based native chemical ligation and its applications in chemical protein synthesis

Fig. 6: Chemical synthesis of ubiquitin (1-76) based on VTANCL.

a The sequence of ubiquitin (1-76); the ligation site is indicated with a dash. b Scheme of the synthesis of ubiquitin (1-76) via a one-pot C-to-N sequential three-segment condensation (six steps in one pot). c Analytical RP-HPLC traces (λ = 214 nm) of each step of the reaction. Peak # indicates interior standard (Benzamide), peak * indicates the hydrolysis byproduct of 8, peak ** indicates product formed by TCEP quenching excess VTT, peak ◆ indicates deprotection product of *, peak ▲ indicates desulfurization product of ◆, 9’ indicates desulfurization product of 9, 11’ indicates desulfurization product of 11. d ESI-MS characterizations of the major products (average isotopes).

Back to article page