Fig. 1: MIDAS identifies putative metabolite ligands of A2AR.
From: Systematic metabolite screening identifies functional regulators of the adenosine A2A receptor
a Schematic of the MIDAS platform. b MIDAS analysis of A2AR. Each data point represents a unique metabolite from the MIDAS metabolite library. Log2(fold change) A2AR-metabolite interactions were normalized to a no protein control dialysis. Dotted lines indicate normalized z-score log2(fold change) > 0.25 and <−0.25, respectively. The MIDAS equilibrium dialysis was performed in triplicate. Statistical significance was calculated using multiple unpaired two-sided t-tests with FDR correction as shown in Supplementary Fig. 2a. c Chemical structures of the prostaglandins. d Dose-response differential scanning fluorimetry of A2AR with the enriched prostaglandins. Data points show mean ± SD of the melting temperature (Tm) at different concentrations. For data points of DMSO and 0.1 mM PGD2, n = 4, for 0.25 mM and 0.5 mM PGD2, n = 5 technical replicates. For all data points for PGE2 and PGI2, n = 3 technical replicates. Data was analyzed using multiple unpaired two-sided t-tests. DMSO vs. 0.25 mM PGD2, p = 0.0365, DMSO vs. 0.5 mM PGD2, p = 0.0018. *p < 0.05, **p < 0.01, ns not significant.
