Fig. 13: Repetitive reversibility of crosslinking reactions: Multiple cycles of disulfide bond formation and cleavage analyzed by PAGE. | Communications Chemistry

Fig. 13: Repetitive reversibility of crosslinking reactions: Multiple cycles of disulfide bond formation and cleavage analyzed by PAGE.

From: Chemical and photoinduced interstrand crosslinking of oligo DNA duplexes containing 2′-deoxythioguanosines

Fig. 13

a, b Chemical oxidation (I2) and reduction with DTT. Conditions: ODN strands (10 µM each), phosphate buffer pH 7.0 (20 mM), NaCl (100 mM), DTT (100 µM), I2 (200 µM), incubated at 10 °C (lane 4), then maintaining DTT:I2 ratios of 2:1 for cleavage and 1:2 for crosslinking (lanes 5–8). c, d Photo-oxidation in the presence of Py–SS–Py followed by DTT reduction. Conditions: ODN strands (10 µM each), phosphate buffer pH 7.0 (20 mM), NaCl (100 mM), Py–SS–Py (100 µM), DTT (100 µM), 365 nm LED light (UV light intensity: 3.0 W/m²) for 10 min at 0 °C.

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