Fig. 5: Thermal denaturation analysis (Tm measurement). | Communications Chemistry

Fig. 5: Thermal denaturation analysis (Tm measurement).

From: Chemical and photoinduced interstrand crosslinking of oligo DNA duplexes containing 2′-deoxythioguanosines

Fig. 5

a Comparison of the native duplex and the TG-containing duplex. b Thermal stability of the TG-containing duplex before and after the crosslinking reaction. Measurement conditions: Tm was determined by monitoring UV absorption at 260 nm from 0 to 90 °C at a heating rate of 1.0 °C/min. [ODN] = 2.0 μM, [NaCl] = 100 mM, in 20 mM phosphate buffer (pH 7.0).

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