Fig. 9: Effect of O2 on photoinduced disulfide crosslinking efficiency. | Communications Chemistry

Fig. 9: Effect of O2 on photoinduced disulfide crosslinking efficiency.

From: Chemical and photoinduced interstrand crosslinking of oligo DNA duplexes containing 2′-deoxythioguanosines

Fig. 9

a Schematic illustration of crosslinking reaction under argon (Ar) atmosphere to evaluate the influence of O2. b PAGE analysis for reaction efficiency under Ar atmosphere versus air-saturated conditions. ODN strands (10 µM each) were prepared in phosphate buffer (20 mM, pH 7.0) containing NaCl (100 mM); photoreaction was then performed using 365 nm LED light (UV light intensity: 3.0 W/m²) for 30 s at 0 °C in an open tube (air-saturated) or in a sealed tube flushed with Ar for 3 or 5 min, followed by irradiation under Ar flow. PAGE analysis condition: 16% acrylamide, 20% formamide, SYBRTM Gold staining, and ChemiDoc MP imaging system. Quantitative analysis of the crosslinked product (% band intensity) showed a marked decrease in reaction efficiency under Ar conditions compared to air-saturated conditions. The crosslinking yield was calculated from the intensity of the crosslinked product band over the sum of the product and remaining single-stranded ODNs.

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