Fig. 4: Stoichiometry and catalytic behavior of RNA–Fe2+ complexes.
From: RNA−Iron complexes catalyse prebiotic oxygen generation

Continuous variation analysis for Fe2+ complexation with A C-puromycin (CPmn), B CCA trinucleotide, and C puromycin (Pmn). The mole fraction of Fe2+ (XFe2+) and RNA (XRNA) ranges from 0 to 1 along the x-axis. UV–Vis absorbance was recorded at 260 nm (OD260), and the data were normalized to OD260 per nucleotide for plotting. The discontinuity point, marked by a dashed arrow, indicates the stoichiometric ratio of Fe2+ to RNA at maximal complex formation. D The oxidative decomposition of H2O2 catalyzed by the CPmn-Fe2+ complex follows enzyme kinetics and is saturable, reaching a plateau at high [H2O2].