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Mind your media

Nature Metabolism volume 2, pages 1369–1372 (2020)Cite this article

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Cell culture media are typically selected on the basis of common laboratory practices but have major effects on the validity, reproducibility and physiological relevance of the scientific findings. We provide arguments and quantitative examples of why choosing an appropriate cell culture medium matters, particularly in metabolic studies.

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Fig. 1: Differences in the concentrations of key metabolic nutrients between human plasma and commonly used culture media modulate intracellular metabolism in vitro.
Fig. 2: Cell culture media significantly affect intracellular metabolite levels in metabolic profiling of the CCLE.

References

  1. Yao, T. & Asayama, Y. Reprod. Med. Biol. 16, 99–117 (2017).

    Article  Google Scholar 

  2. Verma, A., Verma, M. & Singh, A. in Animal Biotechnology 269–293 (Academic Press, 2020).

  3. Lagziel, S., Lee, W. D. & Shlomi, T. BMC Biol. 17, 51 (2019).

    Article  Google Scholar 

  4. Jang, C., Chen, L. & Rabinowitz, J. D. Cell 173, 822–837 (2018).

    Article  CAS  Google Scholar 

  5. Wishart, D. S. et al. Nucleic Acids Res. 46, D608–D617 (2018). (D1).

    Article  CAS  Google Scholar 

  6. Hensley, C. T., Wasti, A. T. & DeBerardinis, R. J. J. Clin. Invest. 23, 3678–3684 (2013).

    Article  Google Scholar 

  7. Davidson, S. M. et al. Cell Metab. 23, 517–528 (2016).

    Article  CAS  Google Scholar 

  8. Muir, A. et al. eLife 6, e27713 (2017).

    Article  Google Scholar 

  9. Birsoy, K. et al. Nature 508, 108–112 (2014).

    Article  CAS  Google Scholar 

  10. Vande Voorde, J. et al. Sci. Adv. 5, eaau7314 (2019).

    Article  Google Scholar 

  11. Cantor, J. R. et al. Cell 169, 258–272.e17 (2017).

    Article  CAS  Google Scholar 

  12. Lien, E. C. & Vander Heiden, M. G. Nat. Rev. Cancer 19, 651–661 (2019).

    Article  CAS  Google Scholar 

  13. Savino, A.M. et al. Nat. Can. https://doi.org/10.1038/s43018-020-00115-2 (2020).

  14. Mandal, R. et al. Genome Med. 30, 38 (2012).

    Article  Google Scholar 

  15. Sullivan, M. R. et al. eLife 8, e44235 (2019).

    Article  CAS  Google Scholar 

  16. Jain, M. et al. Science 336, 1040–1044 (2012).

    Article  CAS  Google Scholar 

  17. Locasale, J. W. Nat. Rev. Cancer 13, 572–583 (2013).

    Article  CAS  Google Scholar 

  18. Maddocks, O. D. K. et al. Nature 493, 542–546 (2013).

    Article  CAS  Google Scholar 

  19. Li, H. et al. Nat. Med. 25, 1–11 (2019).

    Article  Google Scholar 

  20. Lagziel, S., Lee, W. D. & Shlomi, T. BMC Biol. 17, 37 (2019).

    Article  Google Scholar 

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Acknowledgements

T.S. has received funding from European Research Council, ERC grant agreement no. 714738.

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Authors and Affiliations

  1. Faculty of Computer Science, Technion, Haifa, Israel

    Shoval Lagziel & Tomer Shlomi

  2. Ruth and Bruce Rappaport Faculty of Medicine, Technion, Haifa, Israel

    Eyal Gottlieb

  3. Faculty of Biology, Technion, Haifa, Israel

    Tomer Shlomi

  4. Lokey Center for Life Science and Engineering, Technion, Haifa, Israel

    Tomer Shlomi

Authors
  1. Shoval Lagziel
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  2. Eyal Gottlieb
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  3. Tomer Shlomi
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Contributions

S.L. performed the metabolomics data analysis. S.L., E.G. and T.S. wrote the manuscript.

Corresponding authors

Correspondence to Eyal Gottlieb or Tomer Shlomi.

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The authors declare no competing interests.

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Lagziel, S., Gottlieb, E. & Shlomi, T. Mind your media. Nat Metab 2, 1369–1372 (2020). https://doi.org/10.1038/s42255-020-00299-y

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