Extended Data Fig. 2: Effects of STK3/STK4 knockdown or inhibition on the mitochondrial proteins and macroautophagy.
From: STK3/STK4 signalling in adipocytes regulates mitophagy and energy expenditure
a, Immunoblot analysis of adipocytes differentiated from immortalized brown adipocyte precursors transfected with 20 nM Stk3/Stk4 siRNA (n = 4). b, C3H10T1/2 adipocytes were treated with vehicle controls or XMU-MP-1 (XMU) for 24 h and then exposed to chloroquine (50 μg/ml) in nutrient deprivation media (EBSS). LC3II and LC3I levels were detected by western blot (Starv: Starvation, CQ: chloroquine, n = 3). c, Immunoblot analysis of C3H10T1/2 adipocytes transfected with negative controls (N.C.) and siRNA targeting Stk3/Stk4 which were then exposed to chloroquine (50 μg/ml) in nutrient deprivation media (n = 3). Statistical analyses were assessed with unpaired (a) or paired (b,c) two-tailed t-test. Each point represents a biological replicate. Data are presented as the mean ± s.e.m.
