Extended Data Fig. 1: Seahorse analysis of macrophages treated with either IFNγ and LPS or IL-4.

a, Naïve BMDMs were polarized towards a pro-inflammatory phenotype with IFNγ and LPS or a pro-resolving phenotype with IL-4 for 24 h followed by Seahorse analysis. The extracellular acidification rate (ECAR), a measure of glycolysis, was measured at baseline and after the addition of glucose (‘glycolysis’), oligomycin (‘glycolytic capacity’), and 2-DG. The oxygen consumption rate (OCR), a measure of oxidative phosphorylation, was measured at baseline (‘basal respiration’) and after the addition of oligomycin, FCCP (‘maximal respiration), and rotenone plus antimycin A (n = 15-16 wells/group). **P = 0.0017 (naïve vs. IFNγ and LPS), **P = 0.0048 (naïve vs. IL-4), ***P < 0.0001, as compared to the naïve groups. b, The same experiment as described under panel a was performed in HMDMs (n = 7-8 wells/group). *P = 0.046 for glycolysis, *P = 0.044 for glycolytic capacity, *P = 0.021 for basal respiration, as compared to the naïve groups. All values are means ± SEM, and significance was determined by one-way ANOVA with Fisher’s LSD post hoc analysis.